She provided excellent training in laboratory techniques and assisted me in manyįield-sampling trips. Her constant encouragement and advice have helped me troubleshoot in the laboratory and push I want to give special thanks to Andrea Lofano for amazing support throughout this process. Ping Liang for the many discussionsĪnd suggestions to further my research and detailed suggestions for improving the thesis writing. Have helped me get through the tough parts and I’m forever grateful. Have the greatest gratitude for everything that I have learnt from him. He provided me with the utmost support and guidance over the last few years. Jonathan Griffiths for giving me the opportunity to complete Virus and is an emerging concern for stone fruit production. GaIV and ToNSV have neverīeen previously reported in Prunus trees, or in Canada. Multiple ilarviruses were identified in diseased trees including Grapevine associated ilarvirus (GaIV), Tobacco streak virus (TSV), Tomato necrotic spot virus (ToNSV), Prunus necrotic ringspot virus (PNRSV), and Prune dwarf virus (PDV). Identified between the presence of ToRSV and incidence of RPD, suggesting that other factors are Based on these similar symptoms, a total of 507 Prunus trees were surveyedĪnd the frequency of ToRSV infection was determined to be 13%. Tomato ringspot virus (ToRSV) infection can result in similar symptoms to those One viroid were identified in 42 diseased trees, demonstrating a wide diversity of viruses in In diseased trees and characterize viral sequence diversity. Understand the contribution of viruses to RPD, RNA sequencing was employed to identify viruses ![]() The average mortality rate in the Niagara region wasĢ0% among all Prunus species with the highest site-specific mortality rate of 67%. ![]() Our survey showed that diseased trees often showed symptoms of dehydration, leafĬhlorosis, reduced new growth, and death. Were surveyed, along with identification of major viruses in diseased Prunus trees. To better understand RPD, disease symptoms in all major Prunus species in the Niagara region Rapid Prunus Decline (RPD) is an emergingĭisorder resulting in rapid and unexplained death of Prunus species in southern Ontario. Significant reductions in yield and plant death. Plant diseases associated with viral infections are a serious issue for growers, causing
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